OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-SAE1 antibodies

Anti-SAE1 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302053
  • Rabbit Polyclonal Antibody against SAE1 (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401688) , 20ug Explanation
100ug 325 3-7 Days
Add to Shopping Cart
WB(3)
IHC(1)
spacer
Also for SAE1 (NM_005500)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse (Predicted: Rat, Bovine) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1
Alternative NameAOS1; HSPC140; SUA1; UBLE1A
Database LinkNP_005491
Entrez Gene 10055 Human
Entrez Gene 56459 Mouse
Entrez Gene 308384 Rat
FunctionThe dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Related Pathway Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
The AOS1 C-term Pab (Cat. #TA302053) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
WB Image
Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#TA302053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
WB Image
AOS1 Antibody (V315) (Cat. #TA302053) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title