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Home Antibody All anti-SAE1 antibodies

Anti-SAE1 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302053 Rabbit Polyclonal Antibody against SAE1 (C-term) 100ug $325 3-7 Days
LC401688 SAE1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse (Predicted: Rat, Bovine) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:50~100
Predicted MW Explanation 38450 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1
Alternative NameAOS1; HSPC140; SUA1; UBLE1A
Database LinkNP_005491
Entrez Gene 10055 Human
Entrez Gene 56459 Mouse
Entrez Gene 308384 Rat
FunctionThe dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Related Pathway Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
The AOS1 C-term Pab (Cat. #TA302053) is used in Western blot to detect AOS1 in mouse heart tissue lysate.

WB Image
Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#TA302053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).

WB Image
AOS1 Antibody (V315) (Cat. #TA302053) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).

IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.


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