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Home Antibody All anti-SAE1 antibodies

Anti-SAE1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302053
  • Rabbit Polyclonal Antibody against SAE1 (C-term)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401688) , 20ug
100ug $325 3-7 Days
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WB(1)
IHC(1)
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Also for SAE1 (NM_005500)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman, Mouse Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1
Alternative NameAOS1; HSPC140; SUA1; UBLE1A
Database LinkNP_005491
FunctionThe dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
Related Pathway

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WB Image
(LEFT)The AOS1 C-term Pab is used in Western blot to detect AOS1 in mouse heart tissue lysate. (RIGHT)Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
IHC Image
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

 

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