This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human p70 S6Kbeta Antibody.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; IHC: 1:50-100; ELISA: 1: 1,000;
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
Related Pathway
TGF Beta Signaling Pathway
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* OriGene provides validated application data and protocol, with money back guarantee.
(LEFT)Western blot analysis of anti-p70 S6Kbeta Antibody (N-term) Pab in HepG2 cell line lysates (35ug/lane). RPS6KB2(arrow) was detected using the purified Pab (1:60 dilution). (RIGHT)Western blot analysis of anti-p70 S6Kbeta Antibody (N-term) Pab in 293 cell line lysates transiently transfected with the RPS6KB2 gene (2ug/lane). p70 S6Kbeta(arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with p70 S6Kbeta Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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