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Anti-ROBO4 Antibody


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  • Rabbit Polyclonal Antibody against ROBO4 - Tumor Endothelial Marker
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412794) , 20ug Explanation
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OriGene Data

ImmunogenA synthetic peptide made to a C-terminal region of the human protein (within residues 900-1007). [Swiss-Prot# Q8WZ75]
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500
BufferTris-citrate/phosphate [pH 7-8] and 0.1% sodium azide
Purification Immunogen affinity purified

Reference Data

Target NameHomo sapiens roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant 1
Alternative NameECSM4; MRB
Database LinkNP_061928
Entrez Gene 54538 Human
FunctionRoundabouts (ROBO) are cell-surface receptors that mediate repulsive signaling mechanisms at the central nervous system midline. However, ROBOs may also mediate attraction mechanisms in the context of vascular development. ROBO4 is a novel roundabout protein which is restricted in expression to endothelial cells in vitro and sites of angiogenesis in vivo. Because it is not expressed on normal endothelial cells, in vivo, it is a promising tumor endothelial marker.
Related PathwayDruggable Genome

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WB Image
Detection of ROBO4 in HUVEC lysate
IHC Image
Detection of ROBO4 in endothelium and trophoblasts of the placenta
IF Image
Immunocytochemistry/Immunofluorescence: Robo4 antibody was tested in Huvec cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).


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