3-beta hydroxysteroid dehydrogenase (HSD3B1) is a bifunctional enzyme involved in the oxidative conversion of ketosteroids that plays an important role in the synthesis of all steroid hormones. There are two HSD3B1 proteins, designated type I and type II, that are expressed by different genes and function in different areas of the body. HSD3B1 has also been shown to be a highly specific and sensitive trophoblast-associated marker (Mao et. al).
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY HSD3B1 (RC204497) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD3B1.