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Anti-BIRC5 Antibody EPR2675
Also for BIRC5 (NM_001168)
|A synthetic peptide corresponding to residues in human Survivin was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 - 1:1000; IHC-P: 1:100 - 1:250; ICC: 1:100; FC: 1:50
|Does not react with Mouse, Rat. Is unsuitable for IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1|
|Survivin is a unique member of the inhibitor of apoptosis (IAP) protein family that interferes with post-mitochondrial events including activation of caspases. Survivin regulates cell cycle and is expressed in most tumors, but it is barely detectable in the terminally differentiated normal cells and tissues. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target (1). Survivin is expressed in the G2/M phase of the cell cycle. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis (2). |
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Western blot - Anti-Survivin antibody [EPR2675]; All lanes : Anti-Survivin antibody [EPR2675] at 1/500 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MOLT4 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.developed using the ECL technique.Predicted band size : 16 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675]; Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, staining Survivin using ab134170 at a 1/100 dilution.