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Anti-IRF3 Antibody EPR2346


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against IRF3 (Clone EPR2346)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400600) , 20ug Explanation
100ul $325 3-7 Days
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Serine 386 of human IRF3 was used as an immunogen. The antibody only detects IRF3 phosphorylated on Serine 386.
Clone NameEPR2346 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1
Alternative NameMGC94729; interferon regulatory factor 3
Database LinkNP_001562
Entrez Gene 3661 Human
FunctionA family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) (1). IRF-3 is activated through phosphorylation by the kinases IKK epsilon and/or TBK1. Phosphorylation results in IRF-3 dimerization and removal of an autoinhibitory structure to allow interaction with the coactivators CBP/p300. Phosphorylation at Serine 396 through Serine 405 alleviates autoinhibition to allow interaction with CREB-binding protein and facilitates phosphorylation at Serine 385 or Serine 386 (2). Serine 386 is suggested to be the target of the IRF-3 kinase and critical determinant for the activation of IRF-3 (3).
Related PathwayTranscription FactorsDruggable Genome Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - IRF3 (phospho S386) antibody [EPR2346]; All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] at 1/20000 dilution.Lane 1 : Lysate from untreated MCF-7 cells .Lane 2 : Lysate from MCF-7 cells treated with Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labeled goat anti-rabbit at 1/1000 dilution.Predicted band size : 47 kDa.Observed band size : 50 kDa .
Immunocytochemistry/ Immunofluorescence - IRF3 (phospho S386) antibody [EPR2346]; TA301369 staining IRF3 (green) in untreated MCF7 cells by Immunocytochemistry/ Immunofluorescense. Nuclei were counterstained blue with DAPI.


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