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Anti-LGALS3 Antibody EPR2774
Also for LGALS3 (NM_002306)
|A synthetic peptide corresponding to residues in human Galectin-3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000 - 1:10000; FC: 1:30; ICC/IF: 1:100 - 1:250
|Does not react with Mouse. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens lectin, galactoside-binding, soluble, 3 (LGALS3), transcript variant 1|
|CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2|
|Galectins are a family of beta-galactoside-binding animal lectins that are associated with diverse cellular events, including immune response. Glectin-3 has previously been identified in rat, mouse and human tissues and is evolutionarily highly conserved. It is an abundant cell-surface component of inflammatory macrophages and their major non-integrin laminin-binding protein. It is also mainly expressed in colonic epithelial cells. Galectin-3 is a cell surface receptor of the advanced glycosylation end products (AGE), which has involvement in the pathogenesis of nephropathy and other diabetic complications (1-2). |
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Western blot - Galectin 3 antibody [EPR2774]; All lanes : Anti-Galectin 3 antibody [EPR2774] at 1/5000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : SW480 cell lysate.Lane 3 : A431 cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 26 kDa.Observed band size : 30 kDa .
Immunocytochemistry/ Immunofluorescence - Galectin 3 antibody [EPR2774]; ab76466, at a 1/100 dilution, staining Galectin 3 in HeLa cells by Immunofluorescence.