Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1
Synonyms:
ER; Era; ESR; ESRA; NR3A1
Immunogen
A phospho-specific peptide corresponding to residues surrounding Serine 104/106 of human ER alpha was used as an immunogen. The antibody only detects ER alpha phosphorylated on Serine 104/106.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (2). Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Related Pathway
TGF Beta Signaling Pathway
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Western blot analysis on MCF-7 cell lysates using anti-Phospho-ER alpha (pS104/106), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase
