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Home Antibody All anti-BIRC5 antibodies

Anti-BIRC5 Antibody EP2880Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301297
  • Rabbit Monoclonal Antibody against BIRC5/Survivin (Clone EP2880Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400469) , 20ug
100ul $325 In Stock
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WB(1)
IHC(7)
FC(1)
ELISA(1)
Assay(1)
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Also for BIRC5 (NM_001168)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N terminus of human Survivin was used as an immunogen.
Clone NameEP2880Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, ELISA, IHC, FC Suggested DilutionsIHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; WB: 1:5000 - 1:20000; IP: 1:10; FC: 1:50; Sandwich ELISA: Use a concentration of 0.5 ug/ml
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1
Alternative NameAPI4; EPR-1
Database LinkNP_001159
FunctionSurvivin is a unique member of the inhibitor of apoptosis (IAP) protein family that interferes with post-mitochondrial events including activation of caspases. Survivin regulates cell cycle and is expressed in most tumors, but it is barely detectable in the terminally differentiated normal cells and tissues. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target (1). Survivin is expressed in the G2/M phase of the cell cycle. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis (2).
Related Pathway
Apoptosis
Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Survivin antibody [EP2880Y]; All lanes : Anti-Survivin antibody [EP2880Y] at 1/10000 dilution.Lane 1 : HeLa lysates.Lane 2 : Jurkat lysates.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 16 kDa.Observed band size : 16 kDa.
Assay Image
Other-Anti-Survivin antibody [EP2880Y](TA301297); Equilibrium disassociation constant (KD)..
ELISA Image
Sandwich ELISA - Survivin antibody [EP2880Y]; Standard curve for Survivin (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 1ug/ml and Detector Antibody at 0.5ug/ml. Concentration of may vary from lot to lot; please use this curve as guideline.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Survivin antibody [EP2880Y]; TA301297 at 1/250 dilution staining Survivin in human urinary bladder carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Survivin antibody [EP2880Y]; TA301297 at 1/250 dilution staining Survivin in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Survivin antibody [EP2880Y](TA301297); TA301297 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Survivin antibody [EP2880Y](TA301297); TA301297 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Survivin antibody [EP2880Y](TA301297); TA301297 showing positive staining in Fetal kidney tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Survivin antibody [EP2880Y](TA301297); TA301297 showing positive staining in Fetal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Survivin antibody [EP2880Y](TA301297); TA301297 showing positive staining in Normal tonsil tissue.
FC Image
Flow Cytometry-Survivin antibody [EP2880Y](TA301297); Overlay histogram showing HeLa cells stained with TA301297 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized with 0.1% PBS-Tween 20 used under the same conditions.

 

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