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Anti-RAD17 PHOSPHO Antibody EP1519Y
Also for RAD17 (NM_133344)
|A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2500 - 1:5000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 7|
|CCYC; HRAD17; R24L; RAD17SP; RAD24|
|Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3). |
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Western blot - Anti-Rad17 (phospho S645) antibody [EP1519Y]; All lanes : Anti-Rad17 (phospho S645) antibody [EP1519Y] at 1/5000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 77 kDa.