Homo sapiens RAD17 homolog (S. pombe) (RAD17), transcript variant 7
Synonyms:
CCYC; HRAD17; R24L; RAD17SP; RAD24
Immunogen
A phospho-specific peptide corresponding to residues surrounding Serine 645 of human Rad17 was used as immunogen. The antibody only detects Rad17 phosphorylated on Serine 645.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Checkpoint Rad proteins function early in the DNA damage signaling cascade to arrest cell cycle progression in response to DNA damage (1). There is a direct regulatory linkage between the Rad17 homologue and the checkpoint kinases, ATM and ATR (2). ATR but not ATM phosphorylates the Rad17 checkpoint protein on Ser635 and Ser645 in vitro. In undamaged synchronized human cells, these two sites were phosphorylated in late G(1), S, and G(2)/M, but not in early-mid G(1). Treatment of cells with genotoxic stress induced phosphorylation of Rad17 in cells in early-mid G(1). This suggests that ATR and Rad17 are essential components of a DNA damage response pathway in mammalian cells (3).
Related Pathway
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Western blot analysis on HeLa cell lysates using anti-Rad17 phospho (pS645), 1:5000 dilution. Cells were either (A) untreated (B) treated with Lambda Phosphatase.
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