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Anti-FYB Antibody EP2546Y
|A synthetic peptide corresponding to residues on the N-terminus of human FYB was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:5000~10,000; IHC: 1:250~500; ICC: 1: 100~250; FC: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens FYN binding protein (FYB), transcript variant 2|
|ADAP; PRO0823; SLAP-130|
|FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3). Fyn (p59Fyn) is a 59 kDa member of the Src family of tyrosine kinases. The carboxy terminus of Fyn shares extensive amino acid sequence homology with Src, but is very different within the amino-terminal 81 amino acid residues. The Fyn protein is synthesized and N-myristoylated on cytosolic polysomes and then rapidly targeted to the plasma membrane, where it is palmitoylated. Fyn's biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling (2, 3). |
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Western blot - FYB antibody [EP2546Y]; Anti-FYB antibody [EP2546Y] at 1/10000 dilution + TF-1 lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 110 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FYB antibody [EP2546Y]; ab76103, at 1/250 dilution, staining FYB in paraffin-embedded human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - FYB antibody [EP2546Y]; ICC/If image of CHO cell expressing human ADAP using ab 76103. The cells were fixed in formaldehyde and permeabilised using 0.2% Triton X100 for 5 mins. The cells were them blocked using 10% serum for 1 hour.