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Home Antibody All anti-CAPN2 antibodies

Anti-CAPN2 Antibody EPR2562Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301199
  • Rabbit Monoclonal Antibody against CAPN2 (Clone EPR2562Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400662) , 20ug
100ul $325 3-7 Days
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WB(1)
FC(1)
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Also for CAPN2 (NM_001748)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues within human calpain 2 was used as an immunogen.
Clone NameEPR2562Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:2000; IP: 1:20; FC: 1:60
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens calpain 2, (m/II) large subunit (CAPN2), transcript variant 1
Alternative NameCANP2; CANPL2; CANPml; mCANP
Database LinkNP_001739
FunctionThe calcium-activated protease calpain cleaves a variety of biologically important proteins and serves, therefore, as a key regulator of many cellular functions. Activation of both main isoforms, calpain 1 and calpain 2, was demonstrated previously in Alzheimer's disease. Calpain 2 could be directly involved in tau hyperphosphorylation by modulating protein kinase activities. Results provide evidence of the important role of the calpain proteolytic system in the pathogenesis of neurodegenerative diseases with tau neurofibrillary pathology (1). Calpain is intimately involved in modulating cell adhesion and migration. The two ubiquitous isoforms of this protease, calpain I and II, are considered to be cytosolic proteins that can translocate to both focal complexes/adhesions or the plasma membrane. results reveal an increased association of calpain I, II, calpastatin, and the 30kDa regulatory subunit with the endoplasmic reticulum and Golgi apparatus as evidenced by their position in the gradient relative to calnexin, Rab6, caveolin, and beta1 integrin after laminin stimulation. Further experiments established that calpain II colocalizes with phosphatidylinositol 4,5-bisphosphate. (2)
Related Pathway
Focal Adhesion

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WB Image
Western blot - Calpain 2 antibody [EPR2562Y]; Anti-Calpain 2 antibody [EPR2562Y] at 1/500 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Observed band size : 75 kDa .
FC Image
Flow Cytometry - Calpain 2 antibody [EPR2562Y]; Flow cytometric analysis of permeabilized HeLa cells using TA301199 (red) or a rabbit IgG (negative) (green).

 

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