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Anti-CAPN2 Antibody EPR2562Y
Also for CAPN2 (NM_001748)
|A synthetic peptide corresponding to residues within human calpain 2 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000; IP: 1:20; FC: 1:60
|Does not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for ICC or IHC-P.
|Homo sapiens calpain 2, (m/II) large subunit (CAPN2), transcript variant 1|
|CANP2; CANPL2; CANPml; mCANP|
Entrez Gene 824 Human
|The calcium-activated protease calpain cleaves a variety of biologically important proteins and serves, therefore, as a key regulator of many cellular functions. Activation of both main isoforms, calpain 1 and calpain 2, was demonstrated previously in Alzheimer's disease. Calpain 2 could be directly involved in tau hyperphosphorylation by modulating protein kinase activities. Results provide evidence of the important role of the calpain proteolytic system in the pathogenesis of neurodegenerative diseases with tau neurofibrillary pathology (1). Calpain is intimately involved in modulating cell adhesion and migration. The two ubiquitous isoforms of this protease, calpain I and II, are considered to be cytosolic proteins that can translocate to both focal complexes/adhesions or the plasma membrane. results reveal an increased association of calpain I, II, calpastatin, and the 30kDa regulatory subunit with the endoplasmic reticulum and Golgi apparatus as evidenced by their position in the gradient relative to calnexin, Rab6, caveolin, and beta1 integrin after laminin stimulation. Further experiments established that calpain II colocalizes with phosphatidylinositol 4,5-bisphosphate. (2) |
|ProteaseDruggable Genome ApoptosisFocal adhesionAlzheimer's disease|
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Western blot - Calpain 2 antibody [EPR2562Y]; Anti-Calpain 2 antibody [EPR2562Y] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Observed band size : 75 kDa .
Flow Cytometry - Calpain 2 antibody [EPR2562Y]; Flow cytometric analysis of permeabilized HeLa cells using TA301199 (red) or a rabbit IgG (negative) (green).