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Anti-EIF4EBP1 PHOSPHO Antibody EPR2169Y
Also for EIF4EBP1 (NM_004095)
|A phospho specific peptide corresponding to residues surrounding threonine 45 was used as an immunogen. This antibody detects 4E-BP1/2/3 phosophorylated at threonine 45.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000; IHC: 1:50; ICC: 1: 100; IP: 1: 10
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)|
|4E-BP1; 4EBP1; BP-1; PHAS-I|
|4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5 cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4) |
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Western blot - eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]; All lanes : Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y].Lane 1 : 293T cell lysate, untreated.Lane 2 : 293T cell lysate, treated with FBS.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 13 kDa.Observed band size : 14-19 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]; Human breast carcinoma tissue stained with ab68187 at 1/50 dilution.