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Anti-SHC1 PHOSPHO Antibody EPR778(2)Y
Also for SHC1 (NM_183001)
|A phospho specific peptide corresponding to residues surrounding tyrosine 427 of human SHC1 was used as an immunogen. This antibody detects SHC1 phosphorylated on tyrosine 427.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 1|
|The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated form a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type (1-3). Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span (4-5). |
EGFR1 Signaling Pathway
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Western blot - SHC (phospho Y427) antibody [EPR778(2)Y]; All lanes : Anti-SHC (phospho Y427) antibody [EPR778(2)Y] at 1/500 dilution.Lane 1 : A431 lysate .Lane 2 : A431 lysate - EGF treated.Lysates/proteins at 10 ug/ml per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 63 kDa.Observed band size : 66 kDa .Additional bands at : 46 kDa (possible isoform),52 kDa (possible isoform).