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Anti-PBK Antibody EP2519Y
|A synthetic peptide corresponding to residues on the N-terminus of human PBK was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:100000; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens PDZ binding kinase (PBK), transcript variant 1|
|CT84; HEL164; Nori-3; SPK; TOPK|
Entrez Gene 55872 Human
|A novel mitotic kinase, PDZ-binding kinase or PBK, which is upregulated in a variety of neoplasms including hematological malignancies, has been the focus of attention with a goal to understand its role in malignant conversion and to examine as a possible new therapeutic target in disparate types of cancer. Studies provide a plausible explanation for the role of PBK augmenting tumor cell growth following transient appearance in different types of progenitor cells in vivo (1). PBK is a newly identified member of a novel MEK3/6-related MAPKK that may be enrolled in the activation of lymphoid cells and support testicular functions (2). In vitro, PBK binds specifically to PDZ2 of hDlg through its C-terminal T/SXV motif. PBK and hDlg are phosphorylated at mitosis in HeLa cells, and the mitotic phosphorylation of PBK is required for its kinase activity. In vitro, cdc2/cyclin B phosphorylates PBK. This evidence shows how PBK could link hDlg or other PDZ-containing proteins to signal transduction pathways regulating the cell cycle or cellular proliferation (3). |
|Protein KinaseDruggable Genome |
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Western blot - PBK antibody [EP2519Y]; Anti-PBK antibody [EP2519Y] at 1/100000 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 36 kDa.Observed band size : 36 kDa.