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Anti-ACTN1 Antibody EP2527Y
Also for ACTN1 (NM_001102)
|A synthetic peptide corresponding to residues on the N-terminus of human Alpha-Actinin 1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2,000; IHC: 1: 100; FC: 1:80; IP: 1:40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens actinin, alpha 1 (ACTN1), transcript variant 2|
|Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2). |
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Western blot - alpha Actinin antibody [EP2527Y]; All lanes : Anti-alpha Actinin antibody [EP2527Y] at 1/10000 dilution.Lane 1 : NIH 3T3 lysate .Lane 2 : HeLa lysate.Lane 3 : C6 lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 103 kDa.Observed band size : 103 kDa.