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Anti-PRKAB1 Antibody EP2181Y


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against PRKAB1 (Clone EP2181Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416773) , 20ug Explanation
100ul 325 3-7 Days
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Also for PRKAB1 (NM_006253)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 181 of human AMPK beta was used as immunogen.
Clone NameEP2181Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2,000-20,000; IHC: 1: 100; ICC: 1: 100~250
Predicted MW Explanation 30 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1)
Alternative NameAMPK; HAMPKb
Database LinkNP_006244
Entrez Gene 5564 Human
FunctionThe 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising ? (63 kDa), ? (38 kDa) and ? (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). Phsopholyation on serine 24/25 and serine 182 has been linked to AMPK localization.
Related PathwayDruggable Genome Insulin signaling pathwayAdipocytokine signaling pathwayHypertrophic cardiomyopathy (HCM)

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]; All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] at 1/20000 dilution.Lane 1 : human brain tissue lysate (cells were untreated).Lane 2 : human brain tissue lysate (cells were treated with AP).Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 32 and 45 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AMPK beta 1 (phospho S181) antibody [EP2181Y]; Immunohistochemical analysis of paraffin-embedded human skeletal muscle using TA301131 at 1/100-1/250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - AMPK beta 1 (phospho S181) antibody [EP2181Y]; Immunofluorescent staining of HeLa cells using TA301131 at 1/100-1/250 dilution.


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