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Home Antibody All anti-ITGB3 antibodies

Anti-ITGB3 Antibody EPR2417Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301125
  • Rabbit Monoclonal Antibody against ITGB3 (Clone EP2417Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC424861) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(5)
IF(1)
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Also for ITGB3 (NM_000212)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near C-terminus (the cytoplasmic region) of human Integrin ?-3A was used as an immunogen. This antibody does not detect Integrin ?-3B or Integrin ?-3C.
Clone NameEPR2417Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: Use at an assay dependent dilution; IHC-P: 1:100 - 1:250; WB: 1:1000 - 1:2000; IHC-Fr: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) (ITGB3)
Alternative NameBDPLT16; BDPLT2; CD61; GP3A; GPIIIa; GT
Database LinkNP_000203
FunctionPlatelet membrane glycoprotein Integrin ?-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).
Related Pathway
Focal Adhesion
Hematopoietic cell lineage
Hemostasis

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WB Image
Western blot - Anti-Integrin beta 3 antibody [EPR2417Y]; Anti-Integrin beta 3 antibody [EPR2417Y] at 1/2000 dilution + human spleen lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 110 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR2417Y]; TA301125 staining mouse brain sections by IHC-P. The tissue was fixed with paraformaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Permeabilization was performed with 0.1% Tween and blocking of the sample was done with PBS/0.1% Tween/5% goat serumfor1 hourat 25°C, followed by staining with TA301125 at 1/250 in PBS/0.1% Tween/5% goat serum/azide for 12h at 4°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody. Upper panel shows a section from a WT animal and bottom panel shows a section from an animal withfull knockout to the Itgb3 gene. Integrin beta3 staining was observed in NeuN positive cells.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR2417Y]; TA301125 at 1/100 dilution staining Integrin beta 3 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-Integrin beta 3 antibody [EPR2417Y]; TA301125 staining Integrin beta 3 in Human pancreatic PanIN lesion tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% Serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in PBS and incubated with the sample for 8 hours at 4°C. An Alexa Fluor? 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.
IHC Image
Immunohistochemistry (Frozen sections) - Anti-Integrin beta 3 antibody [EPR2417Y]; TA301125 staining Integrin beta 3 in Mouse pancreatic tumor tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with 5% Serum for 1 hour at room temperature. The primary antibody was diluted 1/100 in PBS and incubated with the sample for 8 hours at 4°C. An Alexa Fluor? 488-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 3 antibody [EPR2417Y]; Mouse vessel stained using IHC-P. Recommended dilution for Mouse tissue is 1:100-250.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 3 antibody [EPR2417Y]; Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical SchoolSample: mouse embryonic fibroblastsPreparation:Fix in 3% PFA in PBS for 30 min at RTPrimary antibody: Rabbit anti-integrinbeta3, 1:100Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor? 555) pre-adsorbed , 1:200FITC-phalloidin, 1:100

 

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