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Anti-TP53 PHOSPHO Antibody EP2393Y
Also for TP53 (NM_000546)
|A phospho specific peptide corresponding to residues surrounding serine 33 of human p53 was used as an immunogen. This antibody detects p53 phosphorlyated at serine 53.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:2000; ICC/IF: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P or IP.
|Homo sapiens tumor protein p53 (TP53), transcript variant 1|
|BCC7; LFS1; P53; TRP53|
Entrez Gene 7157 Human
|p53 acts as both a tumor-suppressor and transcription factor that, upon activation by DNA damage and other cellular stress signals, leads to the transcription of genes triggering cell-cycle arrest, apoptosis, and DNA repair (1,2). The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers (3). p53 is frequently mutated or inactivated in about 60% of cancers (4,5). Cyclin dependent kinase 9, whose well-known substrate is RNA polymerase II, can also phosphorylate p53, more specifically, Ser33 on the N-terminus and, Ser315 and Ser392 on the C-terminus (6). |
|Stem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >> |
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Western blot - p53 (phospho S33) antibody [EP2393Y]; All lanes : Anti-p53 (phospho S33) antibody [EP2393Y] at 1/2000 dilution.Lane 1 : T47D cell lysates, untreated.Lane 2 : T47D cell lysates, treated with etoposide.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 53 kDa.Observed band size : 53 kDa.
Immunocytochemistry/ Immunofluorescence - p53 (phospho S33) antibody [EP2393Y]; TA301124 at 1/100 dilution staining phosphorylated p53 in A431 cells by Immunofluorescence.