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Anti-CDH1 PHOSPHO Antibody EP913(2)Y
Also for CDH1 (NM_004360)
|A phospho-specific peptide corresponding to residues surrounding serine 838/840 of human E-cadherin was used as an immunogen. This antibody detects E-cadherin phosphorylated at serine 838 and 840|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:1000; WB: 1:100000 - 1:1e+006; IP: 1:40; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens cadherin 1, type 1, E-cadherin (epithelial) (CDH1)|
|Arc-1; CD324; CDHE; ECAD; LCAM; UVO|
|E-Cadherin, a member of the cadherin superfamily, is implicated as a key player in different cellular processes including development, morphology, polarity, migration and tissue integrity (1-2). E-cadherin is a glycoprotein with an extracellular domain that interacts with other E-cadherin molecules on adjacent cells, thereby establishing adhesion between epithelial cells. Established as a tumor and metastasis suppressor, E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis (3-4). Mutations in E-cadherin are found in gastric, breast, colorectal, thyroid and ovarian cancer (5). |
Wnt Signaling Pathway
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Western blot - E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]; All lanes : Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] at 1/1000000 dilution.Lane 1 : Human brain lysate, untreated.Lane 2 : Human brain lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 97 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76319 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]; Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using ab76319 at 1/100 dilution.
Flow Cytometry - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y]; Overlay histogram showing A431 cells stained with ab76319 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.