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Home Antibody All anti-HSPB1 antibodies

Anti-HSPB1 Antibody EP2293Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301096
  • Rabbit Monoclonal Antibody against HSPB1 (Clone EP2293Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400587) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for HSPB1 (NM_001540)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 15 of human HSP27 was used as immunogen. The antibody will detect HSP27 phosphorylation on Serine 15.
Clone NameEP2293Y IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:2000 - 1:5000; ICC: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,IHC-P or IP.

Reference Data

Target NameHomo sapiens heat shock 27kDa protein 1 (HSPB1)
Alternative NameCMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27
Database LinkNP_001531
Entrez Gene 3315 Human
FunctionHeat shock protein (HSP) 27 is one of the small HSPs, and is involved in stress resistance and actin organization. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells (1,2). HSP27 is phosphorylated at serines 15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (3,4).
Related Pathway MAPK signaling pathwayVEGF signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Hsp27 ( S15) antibody [EP2293Y]; All lanes : Anti-Hsp27 (phospho S15) antibody [EP2293Y] at 1/5000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate, heat treated at 44°C.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit-HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 23 kDa.Observed band size : 27 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Hsp27 ( S15) antibody [EP2293Y]; HeLa cells stained with TA301096 at 1/100 dilution.

 

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