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Anti-DAB2 Antibody EP2297Y
Also for DAB2 (NM_001343)
|A synthetic peptide corresponding to residues near the C-terminus of human Dab2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000 - 1:10000; IP: 1:20; ICC: 1:100 - 1:250; FC: 1:50
|Does not react with Mouse, Rat. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P.
|Homo sapiens Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila) (DAB2), transcript variant 1|
|Dab2 (Disabled homolog 2, Diabled-2) is a mammalian structural homolog of Drosophila Disabled protein and a mitogen-responsive phosphoprotein (1). As a component of the CSF1 signal transduction pathway, Dab2 is normally expressed in ovarian epithelia cells. However, in ovarian carcinoma, Dab2 expression is either lost or down-regulated. Therefore, Dab2 is speculated to be a negative regulator of growth and its down-regulation considered to play critical role in ovarian carcinogenesis (2). |
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - DAB2 antibody [EP2297Y]; Anti-DAB2 antibody [EP2297Y] at 1/2000 dilution + human pancreas tissue lysates at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 105 kDa .
Flow Cytometry-Anti-DAB2 antibody [EP2297Y](ab76253); Overlay histogram showing HeLa cells stained with ab76253 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.