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Anti-SMAD5 PHOSPHO Antibody EP728(2)AY
Also for SMAD5 (NM_005903)
|A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens SMAD family member 5 (SMAD5), transcript variant 1|
|DWFC; JV5-1; MADH5|
|Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8. |
TGF Beta Signaling Pathway
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Western blot - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; All lanes : Anti-SMAD5 (phospho S463 + S465) [EP728(2)AY] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with BMP-4.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 52 kDa.
Immunocytochemistry/ Immunofluorescence - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; Immunofluorescent staining of HeLa cells using TA301089 at a 1/50-1/100 dilution.