Homo sapiens SMAD family member 5 (SMAD5), transcript variant 1
Synonyms:
DWFC; JV5-1; MADH5
Immunogen
A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Smad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.
Related Pathway
TGF Beta Signaling Pathway
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Western blot analysis on HeLa cell lysates using anti-Phospho-Smad5 (pS463/465), 1:500 dilution. Cells were either (A) untreated (B) treated with BMP-4
