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Anti-SMAD5 PHOSPHO Antibody EP728(2)AY
Also for SMAD5 (NM_005903)
|A phospho specific peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5. This antibody detects Smad5 phosphorylated at Serine 463 and 465.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt,IHC-P or IP.
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Western blot - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; All lanes : Anti-SMAD5 (phospho S463 + S465) [EP728(2)AY] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate - untreated.Lane 2 : HeLa cell lysate - treated with BMP-4.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 52 kDa.
Immunocytochemistry/ Immunofluorescence - SMAD5 (phospho S463 + S465) antibody [EP728(2)AY]; Immunofluorescent staining of HeLa cells using TA301089 at a 1/50-1/100 dilution.