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Anti-YAP1 PHOSPHO Antibody EP1675Y


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SKU Description Amount Price Availability*  
TA301075 Rabbit Monoclonal Antibody against YAP1 (Clone EP1675Y) (Phospho-specific) 100ul $325 In Stock
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Ser127 of human YAP65 was used as immunogen. The antibody detects YAP65 phosphorylated at Serine 127.
Clone NameEP1675Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250
BufferpH: 7.20Preservative: 0.01% Sodium azideConstituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purification Protein A purified
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameYes associated protein 1
Alternative NameCOB1|YAP|YAP2|YAP65|YKI
Database LinkNP_006097
Entrez Gene 10413 Human
Entrez Gene 22601 Mouse
Entrez Gene 363014 Rat
FunctionThe transcriptional coactivator Yes-associated protein (YAP, YAP65) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). YAP binds with 14-3-3 after being phosphorylated by Akt at Ser127. Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2)
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - YAP1 (phospho S127) antibody [EP1675Y]; All lanes : Anti-YAP1 (phospho S127) antibody [EP1675Y] at 1/50000 dilution.Lane 1 : HeLa cell lysates, untreated.Lane 2 : HeLa cell lysates, treated with FBS + calyculin.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 65 - 75 kDa.Observed band size : 65 kDa .

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YAP1 (phospho S127) antibody [EP1675Y]; TA301075 at 1/100 dilution staining YAP1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.


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