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Anti-ADD1 Antibody EP2175AY
Also for ADD1 (NM_014189)
|A synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 59.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens adducin 1 (alpha) (ADD1), transcript variant 2|
|Alpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; ? and ?, or ? and ? subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5). |
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Western blot - alpha Adducin (phospho S59) antibody [EP2175AY]; All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] at 1/10000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate, treated with calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labelled IgG at 1/1000 dilution.Predicted band size : 81 kDa.Observed band size : 115 kDa .