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Anti-ADD1 Antibody EP2175AY


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal Antibody against ADD1 (Clone EP2175AY)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser59 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 59.
Clone NameEP2175AY IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:2000; IP: 1:40
Predicted MW Explanation 81 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens adducin 1 (alpha) (ADD1), transcript variant 2
Alternative NameADDA
Database LinkNP_054908
Entrez Gene 118 Human
FunctionAlpha adducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; ? and ?, or ? and ? subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser59, Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Related PathwayDruggable Genome

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Western blot - alpha Adducin (phospho S59) antibody [EP2175AY]; All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] at 1/10000 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate, treated with calyculin A.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labelled IgG at 1/1000 dilution.Predicted band size : 81 kDa.Observed band size : 115 kDa .


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