Novel HIF2A mutations disrupt oxygen sensing, leading to polycythemia, paragangliomas, and somatostatinomas Blood, Mar 2013; 121: 2563 - 2566.
[anti-HA]
Regulation of the PI3-K/Akt Survival Pathway in the Rat Endometrium Biol Reprod, Mar 2013; 88: 79.
[Akt3]
RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt159.
[LA]
Ruxolitinib as potential targeted therapy for patients with JAK2 rearrangements Haematologica, Mar 2013; 98: 404 - 408.
[JAK2]
Homo sapiens spleen tyrosine kinase (SYK), transcript variant 1
Synonyms:
p72-Syk
Immunogen
A phospho specific peptide corresponding to residues surround Tyrosine 323 of human Syk was used as an immunogen. This antibody detects Syk phosphorylated at Tyrosine 323.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Syk (pY323) was found to be critical for the enrichment of the catalytic subunit p110delta of PI3K class I(A) as well as for the generation of PI3K products at the leading edge of the majority of polarized cells (6).
Related Pathway
Hemostasis
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis on U937 cell lysates using anti-Phospho-Syk (pY323), 1:2000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
