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Anti-MAPK10 Antibody EP1597Y
Also for MAPK10 (NM_138982)
|A synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of human JNK3 was used as immunogen. The antibody will detect JNK1 (pY185)/JNK2(pY185)/JNK3 (pY223) phospholyation sites.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:5000 - 1:20000; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 2|
|JNK3; JNK3A; p493F12; p54bSAPK; PRKM10; SAPK1b|
|C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3). |
MAPK signaling pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y]; All lanes : Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] at 1/20000 dilution.Lane 1 : lysates from untreated Hek293 cells.Lane 2 : lysates from Hek293 cells treated with UV.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 48 kDa.Observed band size : 48 kDa.