Anti-ITGA4 Antibody, EPR1355Y

Also for ITGA4 (NM_000885)

OriGene Data

Immunogen	A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha4 was used as an immunogen.
Clone Name	EPR1355Y	Isotype	IgG
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1: 1000 2000; IHC: 1: 100 250; ICC: 1:50; IP: 1:40
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)
Alternative Name	CD49D; IA4
Database Link	NP_000876
Function	VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).
Related Pathway	Focal Adhesion Hematopoietic cell lineage Hemostasis

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WB Image

Western blot analysis on Jurkat cell lysate using anti-VLA-alpha4, dilution 1:2000.

IHC Image

Immunohistochemical staining of paraffin-embedded human tonsils using anti-VLA-alpha4.

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Anti-ITGA4 Antibody EPR1355Y

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data