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Home Antibody All anti-ITGA4 antibodies

Anti-ITGA4 Antibody EPR1355Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300988
  • Rabbit Monoclonal Antibody against ITGA4 (VLA-alpha4) (Clone EPR1355Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400316) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
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Also for ITGA4 (NM_000885)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha4 was used as an immunogen.
Clone NameEPR1355Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:2000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50; IP: 1:40
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)
Alternative NameCD49D; IA4
Database LinkNP_000876
FunctionVLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).
Related Pathway
Focal Adhesion
Hematopoietic cell lineage
Hemostasis

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Integrin alpha 4 antibody [EPR1355Y]; Anti-Integrin alpha 4 antibody [EPR1355Y] at 1/2000 dilution + Jurkat cell lysate at 10 µg.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 150 kDa.Observed band size : 150 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin alpha 4 antibody [EPR1355Y]; Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 TA300988

 

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