Home All anti-ITGA4 antibodies
Anti-ITGA4 Antibody EPR1355Y
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for ITGA4 (NM_000885)
|A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha4 was used as an immunogen.|
||WB: 1: 1000 2000; IHC: 1: 100 250; ICC: 1:50; IP: 1:40
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)|
|VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3). |
Hematopoietic cell lineage
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis on Jurkat cell lysate using anti-VLA-alpha4, dilution 1:2000.
Immunohistochemical staining of paraffin-embedded human tonsils using anti-VLA-alpha4.