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Anti-ITGA4 Antibody EPR1355Y
Also for ITGA4 (NM_000885)
|A synthetic peptide corresponding to residues near the C-terminus of human VLA-alpha4 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:2000; IHC-P: 1:100 - 1:250; ICC/IF: 1:50; IP: 1:40
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)|
|VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3). |
Hematopoietic cell lineage
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Western blot - Integrin alpha 4 antibody [EPR1355Y]; Anti-Integrin alpha 4 antibody [EPR1355Y] at 1/2000 dilution + Jurkat cell lysate at 10 µg.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 150 kDa.Observed band size : 150 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin alpha 4 antibody [EPR1355Y]; Immunohistochemical staining of paraffin-embedded human tonsils using 1/100 ab81280