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Anti-DVL3 Antibody EP1991Y
Also for DVL3 (NM_004423)
|A synthetic peptide corresponding to residues near the C-terminus of human Dsh-3 was used as an immungen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:500 - 1:1000; ICC: 1:100 - 1:250; FC: 1:30; IHC-P: Use at an assay dependent dilution; ICC/IF: Use a concentration of 5 ug/ml
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens dishevelled segment polarity protein 3 (DVL3)|
|The human DVL-3 gene encodes a predicted 716 amino acid protein which exhibits 98% amino acid identity with mouse Dsh-3 and 49% with Drosophila Dsh. Dsh-3 was mapped to 3q27. The expression of Dsh-3 mRNA was detected in 30 human cell lines and 2 primary cell cultures. Dsh-3 mRNA was detected in normal human breast tissues and tumors. Statistically, there was no difference in Dsh-3 mRNA level between normal breast tissues and tumors. In human colorectal samples, Dsh-3 was expressed equally in matched normal tissues, polyps and tumors. The data indicates that Dsh-3 is widely expressed in human cells and may have a widespread role in signal transduction (1). Results indicate that the human dishevelled genes constitute a multigene family and that Dsh 3 proteins are highly conserved among metazoans. |
Wnt Signaling Pathway
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Western blot - Dishevelled 3 antibody [EP1991Y]; Anti-Dishevelled 3 antibody [EP1991Y] at 1/1000 dilution + K562 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled IgG at 1/2000 dilution.Predicted band size : 78 kDa.Observed band size : 95 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dishevelled 3 antibody [EP1991Y]; Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Dishevelled 3 antibody [EP1991Y]; ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-Anti-Dishevelled 3 antibody [EP1991Y](ab76081); Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.