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Home Antibody All anti-PRKD1 antibodies

Anti-PRKD1 PHOSPHO Antibody EP1494Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300975
  • Rabbit Monoclonal Antibody against PRKD1 (Clone EP1494Y) (Phospho-specific)
100uL $325 3-7 Days
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WB(2)
IHC(1)
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Also for PRKD1 (NM_002742)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding S916 were used as an immunogen. This antibody detects PKC mu phosphorylated on Serine 916.
Clone NameEP1494Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50000 - 1:100000; IHC-P: 1:100 - 1:250; IP: 1:60
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens protein kinase D1 (PRKD1)
Alternative NamePKC-MU; PKCM; PKD; PRKCM
Database LinkNP_002733
FunctionPKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control (1). PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. (2). Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain (3).
Related Pathway
EGFR1 Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - Anti-PKC mu (phospho S916) antibody [EP1494Y]; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 102 kDa.Exposure time : 12 minutes
WB Image
Western blot - PKC mu (phospho S916) antibody [EP1494Y]; All lanes : Anti-PKC mu (phospho S916) antibody [EP1494Y] at 1/100000 dilution.Lane 1 : lysate from untreated HeLa cells .Lane 2 : lyaste from HeLa cells treated with TPA.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 102 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC mu (phospho S916) antibody [EP1494Y]; Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 TA300975.

 

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