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Home Antibody All anti-PRNP antibodies

Anti-PRNP Antibody EP1802Y

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300958 Rabbit Monoclonal Antibody against PRNP (Clone EP1802Y) 100uL $325 In Stock
LC403657 PRNP HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(3)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Prp was used as an immunogen.
Clone NameEP1802Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000 - 1:10000; FC: 1:200; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 28 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens prion protein (PRNP), transcript variant 2
Alternative NameAltPrP; ASCR; CD230; CJD; GSS; KURU; p27-30; PRIP; PrP; PrP27-30; PrP33-35C; PrPc
Database LinkNP_898902
Entrez Gene 5621 Human
Entrez Gene 19122 Mouse
Entrez Gene 24686 Rat
FunctionPrion diseases are transmissible neurodegenerative disorders which affect a range of mammalian species. In humans they can be inherited and sporadic as well as acquired by exposure to human prions. Prions appear to be composed principally of a conformational isomer of host-encoded prion protein and propagate by recruitment of cellular prion protein (1). The function of the cellular prion protein (PrP) is still poorly understood. It has been proposed that one unprecedented role for PrP is against Bax-mediated neuronal apoptosis. It has been shown that PrP potently inhibits Bax-induced cell death in human primary neurons (2). An impaired synaptic inhibition may be involved in the epileptiform activity seen in Creutzfeldt-Jakob and other neurodegenerative diseases and it is believed that loss of function of PrP may contribute to the early synaptic loss and neuronal degeneration seen in these diseases (3).
Related PathwayES Cell Differentiation/IPSTransmembraneStem cell - Pluripotency Prion diseases

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Prion protein PrP antibody [EP1802Y]; Anti-Prion protein PrP antibody [EP1802Y] at 1/10000 dilution + fetal brain lysate at 10 ug.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 28 kDa.
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Western blot ; Anti-Prion protein PrP antibody [EP1802Y] at 1/5000 dilution + Mouse Prion protein PrP full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 10 seconds
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Immunohistochemistry (Paraffin-embedded sections) - Prion protein PrP antibody [EP1802Y]; Immunohistochemical analysis of brain glioma using TA300958 at a dilution of 1/100-1/250.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y]; Immunohistochemical analysis of Prion-infected mouse brain tissue, staining Prion protein PrP with TA300958. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) before incubating with primary antibody (1/7000) overnight at 4°C. Staining was detected using DAB.
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Figure from citation: Immunohistochemistry of PRNP protein level by using anti-PRNP antibody in mouse brain tissue sections. Prnp-positive signals were stained brown. +/+: wt mice; -/-: non-treated MPS IIIB mice; AAV9: rAAV9-treated MPS IIIB mice; #1: mouse with low Prnp IHC intensity; #2: mouse with high Prnp IHC intensity; DG: dentate gyrus of hippocampus. PL: polymoph layer of DG. Scale bar: 50 um. View Citation
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Flow Cytometry-Anti-Prion protein PrP antibody [EP1802Y](TA300958); Overlay histogram showing SH-SY5Y cells stained with TA300958 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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