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Anti-KITLG Antibody EP665Y
Also for KITLG (NM_000899)
|A synthetic peptide corresponding to residues near the N-terminus of human SCF was used as an immungen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens KIT ligand (KITLG), transcript variant b|
|FPH2; Kitl; KL-1; MGF; SCF; SF; SHEP7|
|Novel hematopoietic growth factor, stem cell factor (SCF), was cloned and demonstrated to be the ligand for the c-kit tyrosine kinase receptor. SCF is encoded by Sl (steel), a gene critical to the development of several distinct cell lineages during embryonic life and which has important effects on hematopoiesis in the adult (1). SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size (2). |
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
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Western blot - SCF antibody [EP665Y]; Anti-SCF [EP665Y] antibody at 1/10000 dilution + recombinant protein (5ng).Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 18 kDa .
Immunohistochemistry (Paraffin-embedded sections) - SCF antibody [EP665Y]; Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250.
Flow Cytometry - SCF antibody [EP665Y]; Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.