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Anti-YAP1 Antibody EP1674Y
Also for YAP1 (NM_006106)
|A synthetic peptide corresponding to residues near the C-terminus of human YAP65 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:25000 - 1:50000; IP: 1:70; FC: 1:50; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens Yes-associated protein 1 (YAP1), transcript variant 2|
|YAP; YAP2; YAP65; YKI|
|The transcriptional coactivator Yes-associated protein (YAP) has been shown to interact with and to enhance p73-dependent apoptosis in response to DNA damage. It has been shown that YAP requires the promyelocytic leukemia gene (PML) and nuclear body localization to coactivate p73. YAP imparts selectivity to p73 by promoting the activation of a subset of p53 and/or p73 target promoters (1). Akt promotes YAP localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73. p73-mediated induction of Bax expression following DNA damage requires YAP function and is attenuated by Akt phosphorylation of YAP. YAP overexpression increases, while YAP depletion decreases, p73-mediated apoptosis following DNA damage, in an Akt inhibitable manner (2) |
TGF Beta Signaling Pathway
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Western blot - YAP1 antibody [EP1674Y]; Anti-YAP1 antibody [EP1674Y] at 1/50000 dilution + Hela cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 65 kDa.
Western blot - YAP1 antibody [EP1674Y]; .developed using the ECL technique.Predicted band size : 65 kDa.Observed band size : 70 kDa .Exposure time : 1 minuteImage courtesy of an anonymous Abreview.
Immunohistochemistry (Paraffin-embedded sections) - YAP1 antibody [EP1674Y]; Immunohistochemical staining of YAP1 in paraffin embedded human breast carcinoma tissue using ab52771 at a 1/100 dilution.
Flow Cytometry - YAP1 antibody [EP1674Y]; Overlay histogram showing HeLa cells stained with ab52771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.