OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-MCM7 antibodies

Anti-MCM7 Antibody EP1974Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300952
  • Rabbit Monoclonal Antibody against MCM7 (Clone EP1974Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401787) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
IP(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for MCM7 (NM_005916)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human MCM7 was used as an immunogen.
Clone NameEP1974Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsICC/IF: Use a concentration of 1 ug/ml; WB: 1:10000; IP: Use a concentration of 5 ug/ml; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 81 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens minichromosome maintenance complex component 7 (MCM7), transcript variant 1
Alternative NameCDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
Database LinkNP_005907
Entrez Gene 4176 Human
Entrez Gene 17220 Mouse
Entrez Gene 288532 Rat
FunctionThe minichromosome maintenance (MCM7) helicase complex functions to initiate and elongate replication forks. Cell cycle checkpoint signaling pathways regulate DNA replication to maintain genomic stability (1). MCM7 is a putative human homologue of yeast CDC47 and a member of the MCM protein family, which has been implicated in the regulatory machinery causing DNA to replicate only once in the S phase. Findings indicate that MCM7 protein together with other MCM proteins participates in the regulation of mammalian DNA replication (2). In HeLa cells, depletion of MCM7 with small-interfering RNA suppressed ultraviolet (UV) light- or aphidicolin-induced hChk1 phosphorylation, and abolished UV-induced S-phase checkpoint activation. These results demonstrate that MCM7 plays a direct role in the transmission of DNA damage signals from active replication forks to the S-phase checkpoint machinery in human cells (3).
Related PathwayTranscription Factors DNA replicationCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MCM7 antibody [EP1974Y]; Anti-MCM7 antibody [EP1974Y] at 1/10000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 81 kDa.Observed band size : 81 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MCM7 antibody [EP1974Y]; Immunohistochemical analysis of paraffin-embedded human lung tissue using TA300952 at a 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - MCM7 antibody [EP1974Y]; ICC/IF image of TA300952 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody , 1ug/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
IP Image
Immunoprecipitation - Anti-MCM7 antibody [EP1974Y]; MCM7 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit monoclonal to MCM7 and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300952.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 81kDa; MCM7
FC Image
Flow Cytometry - MCM7 antibody [EP1974Y]; Overlay histogram showing HeLa cells stained with TA300952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title