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Anti-ATG7 Antibody EP1759Y
Also for ATG7 (NM_006395)
|A synthetic peptide corresponding to residues near the C-terminus of human Atg7 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||ICC/IF: Use a concentration of 10 ug/ml; WB: 1:100000 - 1:200000; IP: 1:50; ICC: 1:250 - 1:500; FC: 1:50 - 1:100; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens autophagy related 7 (ATG7), transcript variant 1|
|APG7-LIKE; APG7L; GSA7|
Entrez Gene 10533 Human
|Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome/vacuole and has a significant relationship to several neurodegenerative disorders and myopathies in mammals (1). Apg7 (Atg7) is a unique E1 enzyme which is essential for both the Apg12p- and Apg8p-modification systems, and plays indispensable roles in autophagy (2). A new molecular pathway has been defined in which activation of the receptor-interacting protein (a serine-threonine kinase) and Jun amino-terminal kinase induced cell death with the morphology of autophagy. Autophagic death required Atg7 and beclin 1 and was induced by caspase-8 inhibition (3). |
| Regulation of autophagy|
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Western blot - Apg7 antibody [EP1759Y]; Anti-Apg7 antibody [EP1759Y] at 1/500000 dilution + Jurkat cell lysate at 10 ug.Secondary.goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 78 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Apg7 antibody [EP1759Y]; Immunohistochemical analysis of paraffin-embedded human liver using TA300950 at a 1/100 dilution.
Immunocytochemistry/ Immunofluorescence-Apg7 antibody [EP1759Y](TA300950); ICC/IF image of TA300950 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Apg7 antibody [EP1759Y]; Overlay histogram showing HEK293 cells stained with TA300950 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.