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Home Antibody All anti-CD44 antibodies

Anti-CD44 Antibody EPR1013Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300896
  • Rabbit Monoclonal Antibody against CD44 (Clone EPR1013Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400203) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(5)
FC(1)
Assay(1)
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Also for CD44 (NM_000610)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human CD-44 was used as an immunogen.
Clone NameEPR1013Y IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, FC Suggested DilutionsIHC-P: 1:50 - 1:100; IHC-Fr: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; WB: 1:5000; FC: 1:30
Predicted MW Explanation 82 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens CD44 molecule (Indian blood group) (CD44), transcript variant 1
Alternative NameCDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1
Database LinkNP_000601
Entrez Gene 960 Human
Entrez Gene 12505 Mouse
FunctionCD44 is a cell-surface glycoprotein postulated to play a role in a variety of biological processes, including lymphocyte homing and tumor-cell metastasis. Several isoforms of CD44 have been identified in human cells, and the genesis of some of these isoforms has been attributed to alternative splicing. Understanding of mechanisms regulating CD44 alternative splicing may provide insights into diverse processes, including tumor-cell metastasis and lymphocyte homing (1). The deduced protein sequence of 699 amino acids has a novel 339 amino acid domain inserted into the proximal extracellular domain of the standard, leukocyte form of CD44. The additional domain adds a number of potential N- and O-linked glycosylation sites and two proteolysis sites to this form of CD44 (2). CD44 is defined as a structurally diverse, but immunologically related, set of intrinsic membrane macromolecules, and suggests that these structurally varied forms might be expected to manifest multiple functions (3)
Related PathwayStem cell relevant signaling - DSL/Notch pathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsCancer stem cellsEmbryonic stem cellsDruggable Genome ECM-receptor interactionHematopoietic cell lineage

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - CD44 antibody [EPR1013Y]; Anti-CD44 antibody [EPR1013Y] at 1/5000 dilution + TF-1 cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 82 kDa.
Assay Image
Other-Anti-CD44 antibody [EPR1013Y](TA300896); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - CD44 antibody [EPR1013Y]; Ab51037 (1:100) staining human CD44 in human urinary bladder tissue by immunohistochemistry using paraffin embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD44 antibody [EPR1013Y](TA300896); TA300896 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD44 antibody [EPR1013Y](TA300896); TA300896 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-CD44 antibody [EPR1013Y](TA300896); TA300896 showing positive staining in Thyroid gland carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y]; TA300896 showing positive staining in Normal tonsil tissue.
FC Image
Flow Cytometry - Anti-CD44 antibody [EPR1013Y]; TA300896 used at a 1/30 dilution to detect CD44 in human MDA-MB-231 cells by Flow Cytometry. The secondary used was a Phycoerythrin conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.The purple plot indicates IgG isotype control and the green plot represents CD44.

 

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