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Home Antibody All anti-ERBB2 antibodies

Anti-ERBB2 Antibody EP1046Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300890
  • Rabbit Monoclonal Antibody against ERBB2 (Clone EP1448Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417979) , 20ug
100ul 325 3-7 Days
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WB(2)
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Also for ERBB2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 1139 of human HER2 was used as an immunogen.
Clone NameEP1046Y IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:500 - 1:1000; IP: 1:30; FC: 1:10
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC/IF,IHC-Fr or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC/IF,IHC-Fr or IHC-P.

Reference Data

Target NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
Alternative NameCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Database LinkNP_004439
FunctionHER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins (2). Overexpression of HER2 is detected in almost 40% of human breast cancers (3). Therefore, HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation (4). Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha (5).
Related Pathway
Adherens junction
Focal Adhesion

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WB Image
Western blot - ErbB 2 (phospho Y1139) antibody [EP1448Y]; All lanes : Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] at 1/1000 dilution.Lane 1 : SKBR3.Lane 2 : SKBR3 + EGF cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 185 kDa .
WB Image
Western blot - ErbB 2 (phospho Y1139) antibody [EP1448Y]; All lanes : Anti-ErbB 2 (phospho Y1139) antibody [EP1046Y] at 1/2000 dilution.Lane 1 : HTB-2O Human breast ductal carcinoma lysate untreated.Lane 2 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 3 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 4 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 5 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 6 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 7 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 8 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lane 9 : HTB-2O Human breast ductal carcinoma lysate treated with HER-2 inhibitor.Lysates/proteins at 10 µg per lane.Secondary.HRP-conjugated Mouse anti-Rabbit monoclonal at 1/20000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 138 kDa.Exposure time : 5 secondsThis image is courtesy of an Abreview by Surekha Pimple.

 

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