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Anti-PRKD2 Antibody EP1495Y
Also for PRKD2 (NM_016457)
|A synthetic peptide corresponding to residues within human PKD2 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; FC: 1:20; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens protein kinase D2 (PRKD2), transcript variant 1|
|HSPC187; nPKC-D2; PKD2|
|Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester- and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2). |
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Western blot - Protein Kinase D2 antibody [EP1495Y]; Anti-Protein Kinase D2 antibody [EP1495Y] at 1/500 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 97 kDa.Observed band size : 105 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Protein Kinase D2 antibody [EP1495Y]; Ab51250 (1:100) staining human Protein Kinase D2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.