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Home Antibody All anti-KRT19 antibodies

Anti-KRT19 Antibody EP1580Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300867
  • Rabbit Monoclonal Antibody against KRT19 (Clone EP1580Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419428) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(6)
IF(2)
FC(1)
Assay(1)
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Also for KRT19 (NM_002276)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human CK-19 was used as an immunogen.
Clone NameEP1580Y IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:50000 - 1:200000; IP: 1:60; ICC: 1:250 - 1:500; FC: 1:100; IHC-P: Use at an assay dependent concentration
Predicted MW Explanation 44 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens keratin 19, type I (KRT19)
Alternative NameCK19; K19; K1CS
Database LinkNP_002267
Entrez Gene 3880 Human
Entrez Gene 16669 Mouse
FunctionCytokeratin polypeptide 19 (CK19) is a type I intermediate filament protein that is expressed in stratified and simple-type epithelia (1). Cytokeratins 8 and 19 concentrate at costameres of striated muscle and copurify with the dystrophin-glycoprotein complex, perhaps through the interaction of the cytokeratins with the actin-binding domain of dystrophin. Studies in COS-7 cells and in vitro showed that dystrophin binds directly and specifically to K19. Results show that dystrophin interacts through its ABD with CK19 specifically and are consistent with the idea that cytokeratins associate with dystrophin at the sarcolemma of striated muscle (2). CK19 is synthesized mainly in embryonic and adult simple epithelia, but has also been found in stratified epithelia as well. CK19 is the smallest known keratin and is remarkable in that, contrary to all other keratins, it does not have a designated partner for the formation of filaments, implying that regulation of its expression is different from other keratin-encoding genes (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Cytokeratin 19 antibody [EP1580Y]; Anti-Cytokeratin 19 antibody [EP1580Y] at 1/1000000 dilution + HepG2 cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 44 kDa.Observed band size : 35 kDa .
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Other-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 19 antibody [EP1580Y]; TA300867, at a 1/250 dilution, staining human Cytokeratin 19 in Kidney carcinoma by Immunohistochemistry, Paraffin embedded tissue.
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Immunohistochemistry (Paraffin-embedded sections) - Cytokeratin 19 antibody [EP1580Y]; TA300867, at a 1/250 dilution, staining human Cytokeratin 19 in Hepatocellular carcinoma by Immunohistochemistry, Paraffin embedded tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); TA300867 showing positive staining in Breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); TA300867 showing positive staining in Endometrial carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); TA300867 showing negative staining in Glioma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); TA300867 showing positive staining in Gastric adenocarcinoma tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [EP1580Y]; TA300867 stainingCytokeratin 19inHuman iPSCderived neural stem cell progenitors by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with4% paraformaldehyde, permeabilized with0.1% Saponin buffer and blocked with 5%Serum for 30 minutes at 20°C. Samples were incubated with primary antibody (5ug/ml in0.1% Saponin buffer) for8 hours at 4°C. An Alexa Fluor?488-conjugatedGoat anti-rabbit IgG polyclonal (1/500)was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Cytokeratin 19 antibody [EP1580Y]; TA300867 stained HeLa cells
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Flow Cytometry-Anti-Cytokeratin 19 antibody [EP1580Y](TA300867); Overlay histogram showing HeLa cells stained with TA300867 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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