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Anti-TUBB3 Antibody EP1569Y
Also for TUBB3 (NM_006086)
|A synthetic peptide corresponding to the C-term of human class III ß-Tubulin was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:40; ICC: 1:100 - 1:250; FC: 1:10; IHC-P: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tubulin, beta 3 class III (TUBB3), transcript variant 1|
|beta-4; CDCBM; CDCBM1; CFEOM3A; TUBB4|
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3). |
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Western blot - Anti-beta III Tubulin antibody [EP1569Y]; Anti-beta III Tubulin antibody [EP1569Y] at 1/10000 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution
Immunohistochemistry (Paraffin-embedded sections) - beta Tubulin antibody [EP1569Y]; ab52623, at a 1/50 dilution, staining human class III beta Tubulin in breast carcinoma tissue using Immunohistochemistry, Paraffin embedded tissue.