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Anti-CASP6 Antibody EP1325Y
Also for CASP6 (NM_001226)
|A synthetic peptide corresponding to residues of human Caspase-6 was used as immunogen. The antibody should recognize the pro-form of Caspase-6.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:20,000; IHC: 1: 100 250; ICC: 1: 100 250; FC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens caspase 6, apoptosis-related cysteine peptidase (CASP6), transcript variant alpha|
|Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-6 is a major apoptotic executioner (1,2). It exists as a pro-domain protein and is cleaved by caspase-3 into two active subunits, an 18 kDa and a 10 kDa subunit (3). Caspase-6 cleaves among many different intracellular proteins, nuclear lamins. Lamins play a significant role in maintaining the integrity of the cell membrane, and their cleavage results in subsequent cellular breakdown and death (4). |
MAPK signaling pathway
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Western blot - Caspase 6 antibody [EP1325Y]; Anti-Caspase 6 antibody [EP1325Y] at 1/20000 dilution + MCF-7 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 34 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Caspase 6 antibody [EP1325Y]; ab52951 at 1/100 dilution staining Caspase 6 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Caspase 6 antibody [EP1325Y]; ab52951 at 1/100 dilution staining Caspase 6 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Caspase 6 antibody [EP1325Y]; Overlay histogram showing Hela cells stained with ab52951 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.