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Anti-APOA1 Antibody EP1368Y
Also for APOA1 (NM_000039)
|A synthetic peptide corresponding to residues near the N-terminus of human Apo A1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||Sandwich ELISA: 1:2000 - 1:4000; WB: 1:20000; IP: 1:60; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens apolipoprotein A-I (APOA1)|
|Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3). |
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Western blot - Apolipoprotein A I antibody [EP1368Y]; Anti-Apolipoprotein A I antibody [EP1368Y] at 1/20000 dilution + fetal liver lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 31 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Apolipoprotein A I antibody [EP1368Y]; ab52945 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Apolipoprotein A I antibody [EP1368Y]; ab52945 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.