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Anti-APOA1 Antibody EP1368Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300821 Rabbit Monoclonal Antibody against APOA1 (Clone EP1368Y) 100ul $325 In Stock
LC400009 APOA1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for APOA1 (NM_000039)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Apo A1 was used as an immunogen.
Clone NameEP1368Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsSandwich ELISA: 1:2000 - 1:4000; WB: 1:20000; IP: 1:60; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens apolipoprotein A-I (APOA1), transcript variant 1
Alternative NameMGC117399
Database LinkNP_000030
Entrez Gene 335 Human
FunctionApolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Related PathwaySecreted ProteinDruggable Genome PPAR signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Apolipoprotein A I antibody [EP1368Y]; Anti-Apolipoprotein A I antibody [EP1368Y] at 1/20000 dilution + fetal liver lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 31 kDa.

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Apolipoprotein A I antibody [EP1368Y]; TA300821 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.

IF Image
Immunocytochemistry/ Immunofluorescence - Apolipoprotein A I antibody [EP1368Y]; TA300821 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.


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