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Home Antibody All anti-APOA1 antibodies

Anti-APOA1 Antibody EP1368Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300821
  • Rabbit Monoclonal Antibody against APOA1 (Clone EP1368Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400009) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for APOA1 (NM_000039)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Apo A1 was used as an immunogen.
Clone NameEP1368Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsSandwich ELISA: 1:2000 - 1:4000; WB: 1:20000; IP: 1:60; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens apolipoprotein A-I (APOA1)
Alternative NameMGC117399
Database LinkNP_000030
FunctionApolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).
Related Pathway
Hemostasis

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WB Image
Western blot - Apolipoprotein A I antibody [EP1368Y]; Anti-Apolipoprotein A I antibody [EP1368Y] at 1/20000 dilution + fetal liver lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 31 kDa.Observed band size : 31 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Apolipoprotein A I antibody [EP1368Y]; TA300821 at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Apolipoprotein A I antibody [EP1368Y]; TA300821 at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.

 

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