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Home Antibody All anti-GYS1 antibodies

Anti-GYS1 Antibody EP852Y

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300820
  • Rabbit Monoclonal Antibody against GYS1 (Clone EP852Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400769) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for GYS1 (NM_002103)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser641 of human Glycogen Synthase was used as immunogen. The antibody only detects Glycogen Synthase phosphorylated on Serine 641.
Clone NameEP852Y IsotypeTissue culture supernatant
Species ReactivityHuman, Mouse ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; IP: 1:60
BufferDoes not react with Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1
Alternative NameGSY; GYS
Database LinkNP_002094
FunctionGlycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-?-D-glucosyl)n to yield UDP and (1,4-?-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.
Related Pathway

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WB Image
Western blot - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] at 1/10000 dilution.Lane 1 : Mouse muscle lysate, untreated.Lane 2 : Mouse muscle lysate, treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; Immunohistochemical analysis of Human liver tissue, using 1/100 TA300820
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y]; ICC/IF image of TA300820 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

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