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Anti-GYS1 Antibody EP852Y
Also for GYS1 (NM_002103)
|A synthetic phospho-peptide corresponding to residues surrounding Ser641 of human Glycogen Synthase was used as immunogen. The antibody only detects Glycogen Synthase phosphorylated on Serine 641.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; IP: 1:60
|Does not react with Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1|
|Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-?-D-glucosyl)n to yield UDP and (1,4-?-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b. |
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Western blot - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] at 1/10000 dilution.Lane 1 : Mouse muscle lysate, untreated.Lane 2 : Mouse muscle lysate, treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230
Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y]; ICC/IF image of ab81230 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.