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Anti-CYCS Antibody EP1326Y
Also for CYCS (NM_018947)
|A synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IP: 1:50; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens cytochrome c, somatic (CYCS)|
|CYC; HCS; THC4|
|Cytochrome C is located in the mitochondria of all aerobic cells and is involved in the electron transport system that functions in oxidative phosphorylation. It accepts electrons from cytochrome b and transfers them to cytochrome oxidase. In the process, the iron of the heme group, which is identical to that of hemoglobin and myoglobin, shifts from the ferrous to the ferric state (1). Recent progress in studies on apoptosis has revealed that cytochrome C is a pro-apoptotic factor. It is released from its places on the outer surface of the inner mitochondrial membrane at early steps of apoptosis and, combining with some cytosolic proteins, activates conversion of the latent apoptosis-promoting protease pro-caspase-9 to its active form. Cytochrome C release can be initiated by the pro-apoptotic protein Bax. This process is blocked by the anti-apoptotic proteins Bcl-2 and Bcl-xL (2). Also, differences in amino acid composition of cytochrome c have also been used as markers of biochemical evolution. |
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Western blot - Cytochrome C antibody [EP1326Y]; All lanes : Anti-Cytochrome C antibody [EP1326Y] at 1/200 dilution.Lane 1 : HeLa cell lysate.Lane 2 : COS cell lysate.Lane 3 : L929 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 12 kDa.Observed band size : 15 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytochrome C antibody [EP1326Y]; ab76107 at 1/100 dilution staining Cytochrome C in human kidney by Immunohistochemistry using paraffin-embedded tissue.