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Anti-TUBB3 Antibody EP1331Y
Also for TUBB3 (NM_006086)
|A synthetic peptide corresponding to residues near the C-term of human ß-Tubulin was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:20000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:10 - 1:100; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens tubulin, beta 3 class III (TUBB3), transcript variant 1|
|beta-4; CDCBM; CDCBM1; CFEOM3A; TUBB4|
|Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3). |
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Western blot - beta III Tubulin antibody [EP1331Y]; Anti-beta III Tubulin antibody [EP1331Y] at 1/20000 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Western blot - beta III Tubulin antibody [EP1331Y]; Anti-beta III Tubulin antibody [EP1331Y] at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 µg.Secondary.An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 50 kDa.Observed band size : 55 kDa .Exposure time : 45 secondsThis image is courtesy of an anonymous Abreview
Immunohistochemistry (Paraffin-embedded sections) - beta III Tubulin antibody [EP1331Y]; ab52901 at 1/250 dilution staining beta III Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - beta III Tubulin antibody [EP1331Y]; ab52901 at 1/100 dilution staining beta III Tubulin in HeLa cells by Immunofluorescence.
Flow Cytometry-beta III Tubulin antibody [EP1331Y](ab52901); Overlay histogram showing HeLa cells stained with ab52901 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.