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Home Antibody All anti-TUBB3 antibodies

Anti-TUBB3 Antibody EP1331Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300786
  • Rabbit Monoclonal Antibody against TUBB3 (Clone EP1331Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416872) , 20ug Explanation
100ul 325 In Stock
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WB(2)
IHC(1)
IF(1)
FC(1)
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Also for TUBB3 (NM_006086)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human ß-Tubulin was used as immunogen.
Clone NameEP1331Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:20000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:10 - 1:100; IHC-P: Use at an assay dependent dilution
Predicted MW Explanation 50 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens tubulin, beta 3 class III (TUBB3), transcript variant 1
Alternative Namebeta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4
Database LinkNP_006077
Entrez Gene 10381 Human
Entrez Gene 22152 Mouse
Entrez Gene 246118 Rat
FunctionMicrotubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers (3).
Related PathwayES Cell Differentiation/IPSDruggable Genome Gap junctionPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - beta III Tubulin antibody [EP1331Y]; Anti-beta III Tubulin antibody [EP1331Y] at 1/20000 dilution + HeLa cell lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
WB Image
Western blot - beta III Tubulin antibody [EP1331Y]; Anti-beta III Tubulin antibody [EP1331Y] at 1/1000 dilution (in PBS +0.5% Tween20 for 2 hours at 23°C) + 293 human embryonic kidney whole cell lysate at 25 ug.Secondary.An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 50 kDa.Observed band size : 55 kDa .Exposure time : 45 seconds
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - beta III Tubulin antibody [EP1331Y]; TA300786 at 1/250 dilution staining beta III Tubulin in human gastric carcinoma by Immunohistochemsitry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - beta III Tubulin antibody [EP1331Y]; TA300786 at 1/100 dilution staining beta III Tubulin in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry-beta III Tubulin antibody [EP1331Y](TA300786); Overlay histogram showing HeLa cells stained with TA300786 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

 

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