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Anti-SYP Antibody EP1098Y
|A synthetic peptide corresponding to residues in human Synaptophysin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||IHC-P: Use at an assay dependent dilution; WB: 1:1000 - 1:10000; IP: 1:50 - 1:100; FC: 1:20 - 1:100; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens synaptophysin (SYP)|
|Synaptophysin is an integral membrane protein of small synaptic vesicles in brain and endocrine cells (1). Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a number of nerve terminal functions (2). Synaptophysin is one of the most abundant membrane proteins of small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. It has been shown that synaptobrevin is preferentially cleaved by tetanus toxin while bound to synaptophysin or when existing as a homodimer (3). data suggest that synaptophysin is released from the Golgi apparatus in a vesicular form, after glycosylation, and is then transported to nerve endings by a mechanism which requires integrity of microtubules (4). |
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Western blot - Synaptophysin antibody [EP1098Y]; Anti-Synaptophysin antibody [EP1098Y] at 1/200000 dilution + Rat brain membrane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 38 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Synaptophysin antibody [EP1098Y]; Human medulloblastoma labelled with ab52636 at 1/250 - 1/500 dilution
Immunocytochemistry - Synaptophysin antibody [EP1098Y]; PC12 cells labelled with ab52636 at 1/250 - 1/500 dilution
Flow Cytometry - Synaptophysin antibody [EP1098Y]; Overlay histogram showing PC12 cells stained with ab52636 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.