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Anti-MUC1 Antibody EP1024Y
Also for MUC1 (NM_002456)
|A synthetic peptide corresponding to residues near the N-terminus of human MUC1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||IHC-Fr: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; FC: 1:30; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF.
|Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 1|
|CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM|
|Mucins are high molecular weight glycoproteins that provide a protective layer on epithelial surfaces and are involved in cell-cell interactions, signaling, and metastasis. MUC1 consists of an extracellular subunit and a membrane-associated subunit (1). The two moieties are produced from a single precursor polypeptide by an early proteolytic cleavage event but remain associated throughout intracellular processing and transport to the cell surface. It has been shown that MUC1 expression inhibits erbB1 (regulators of cell survival and growth) degradation and also E-cadherin (2). Isoform 7 of MUC1 behaves as a receptor and binds the secreted isoform 5. The binding induces the phosphorylation of the isoform 7, alters cellular morphology and initiates cell signaling (3). |
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Western blot - MUC1 antibody [EP1024Y]; Anti-MUC1 antibody [EP1024Y] at 1/1000 dilution + T47D cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labelled, at 1/2000 dilution.Observed band size : 22 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MUC1 antibody [EP1024Y]; Ab45167 at a 1/100 dilution, staining human MUC1 in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunohistochemistry (Frozen sections) - Anti-MUC1 antibody [EP1024Y]; Ab45167 staining MUC1 on E14.5 mouse gut epithelium by IHC-Fr. The tissue was formaldehyde fixed and then blocked for 1 hour at 25°C. The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor 488 conjugated goat anti-rabbit antibody was used as the secondary.