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Anti-CRK Antibody EP242Y
|A synthetic peptide corresponding to residues in the second SH3 domain and near C-terminus of human Crk (p38) protein was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 10,000; ICC: 1:250-500; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens v-crk avian sarcoma virus CT10 oncogene homolog (CRK), transcript variant II|
|Crk (p38) are adaptor proteins, composed mostly of SH2 and SH3 domains, which play a key role in cellular signaling by mediating the construction of protein complexes and also by managing cell proliferation, differentiation, and migration (1). They are critical integrators of upstream signals for cell invasion and migration in human cancer cell lines. Crk is believed to be a regulator of invasive responses because increased levels of the protein have been observed in multiple human cancers (2). In vivo studies have demonstrated that decreased levels of Crk remarkably inhibits tumor formation and its invasive growth (3). Crk associates with ERM family proteins (including ezrin, radixin, and moesin), activates RhoA, and promotes cell motility toward hyaluronic acid (4). |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - Crk p38 antibody [EP242Y]; Anti-Crk p38 antibody [EP242Y] at 1/10000 dilution + HepG2 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 34 kDa.Observed band size : 37 kDa .
Immunocytochemistry/ Immunofluorescence - Crk p38 antibody [EP242Y]; Immunofluorescent staining of HeLa cells using ab45136 at a 1/250 dilution.