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Home Antibody All anti-EZR antibodies

Anti-EZR Antibody EP886Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300648
  • Rabbit Monoclonal Antibody against EZR (Clone EP886Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401164) , 20ug
100ul $325 In Stock
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WB(1)
IHC(6)
IF(1)
FC(1)
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Also for EZR (NM_003379)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Ezrin was used as immunogen.
Clone NameEP886Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:50 - 1:100; WB: 1:5000 - 1:50000; IP: 1:50; FC: 1:1000; IHC-P: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens ezrin (EZR), transcript variant 1
Alternative NameCVIL; CVL; HEL-S-105; VIL2
Database LinkNP_003370
FunctionErzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Phosphorylation at threonine 567 is a critical regulator of ezrin function allowing the active protein to link target molecules to the actin cytoskeleton (4). Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho (5).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ezrin antibody [EP886Y]; Anti-Ezrin antibody [EP886Y] at 1/50000 dilution + Hela cell lysate at 10 µg.Predicted band size : 69 kDa.Observed band size : 72 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Ezrin antibody [EP886Y]; TA300648 at a 1:100 dilution staining Ezrin in human colon carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ezrin antibody [EP886Y](TA300648); Fluorescent immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using TA300648. Green-Ezrin red-PI.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ezrin antibody [EP886Y](TA300648); TA300648 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ezrin antibody [EP886Y](TA300648); TA300648 showing positive staining in Prostatic carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ezrin antibody [EP886Y](TA300648); TA300648 showing positive staining in Hepatocellular carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ezrin antibody [EP886Y](TA300648); TA300648 showing positive staining in Normal kidney tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y]; ICC/IF image of TA300648 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA300648 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-Ezrin antibody [EP886Y]; Overlay histogram showing SH-SY5Y cells stained with TA300648 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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