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Anti-CALM2 Antibody EP799Y
Also for CALM2 (NM_001743)
|A synthetic peptide corresponding to residues in the C-terminus of human Calmodulin was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:50; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens calmodulin 2 (phosphorylase kinase, delta) (CALM2)|
|CAMII; PHKD; PHKD2|
|Calmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).|
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Western blot - Calmodulin antibody [EP799Y]; Anti-Calmodulin antibody [EP799Y] at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane).Predicted band size : 17 kDa.Observed band size : 16 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Calmodulin antibody [EP799Y]; Ab45689 (1:250) staining human Calmodulin in human urinary bladder carcinoma by immunohistochemistry in paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Calmodulin antibody [EP799Y]; ICC/IF image of TA300636 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry - Anti-Calmodulin antibody [EP799Y]; Overlay histogram showing MCF-7 cells stained with TA300636 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.