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Home Antibody All anti-CALM2 antibodies

Anti-CALM2 Antibody EP799Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300636 Rabbit Monoclonal Antibody against CALM2 (Clone EP799Y) 100ul $325 In Stock
LC400660 CALM2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for CALM2 (NM_001743)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of human Calmodulin was used as immunogen.
Clone NameEP799Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:100 - 1:250; FC: 1:50; IP: 1:50
Predicted MW Explanation 17 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target Namecalmodulin 2 (phosphorylase kinase, delta)
Alternative NameCAMII|LQT15|PHKD|PHKD2|caM
Database LinkNP_001734
Entrez Gene 805 Human
Entrez Gene 12314 Mouse
Entrez Gene 50663 Rat
FunctionCalmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).
Related PathwayDruggable Genome Calcium signaling pathwayPhosphatidylinositol signaling systemOocyte meiosisVascular smooth muscle contractionLong-term potentiationNeurotrophin signaling pathwayMore Pathways >>

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WB Image
Western blot - Calmodulin antibody [EP799Y]; Anti-Calmodulin antibody [EP799Y] at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane).Predicted band size : 17 kDa.Observed band size : 16 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Calmodulin antibody [EP799Y]; Ab45689 (1:250) staining human Calmodulin in human urinary bladder carcinoma by immunohistochemistry in paraffin embedded tissue.

IF Image
ICC/IF image of TA300636 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

FC Image
Flow Cytometry - Anti-Calmodulin antibody; Overlay histogram showing MCF-7 cells stained with TA300636 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


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